Journal: Separation and Purification Technology
Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
doi: 10.1016/j.seppur.2024.126567
Figure Lengend Snippet: Fig. 4. Ferroptosis in synergetic treatment. (a) Cell viability of U14 cells preincubated with DMSO or Fer-1 and following different treatments. (b) Intracellular iron content in U14 cells following different treatments. (c) GSH consumption in U14 cells following different treatments. (d) Intracellular MDA level. (e) Flow cytometric assay of LPO and (f) fluorescence microscope images of BODIPY 581/591 staining in U14 cells following different treatments (Scale bar: 200 μm). (g) Western blotting images for the expressions of typical proteins associated with ferroptosis. Semi-quantitative analysis of the expression of (h) SLC7A11, (i) GPX4, (j) ACSL4, and (k) PTGS2 in U14 cells after different treatments.
Article Snippet: Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster.
Techniques: Flow Cytometry, Fluorescence, Microscopy, Staining, Western Blot, Expressing